What is the catalog number for Rabbit anti-14-3-3 beta Antibody?

The catalog number for Rabbit anti-14-3-3 beta Antibody is YLD0001.

What sizes are available for Rabbit anti-14-3-3 beta Antibody?

Rabbit anti-14-3-3 beta Antibody is available in the following size(s): 50 µL, 100 µL.

How many pack sizes does Rabbit anti-14-3-3 beta Antibody come in?

Rabbit anti-14-3-3 beta Antibody is available in 2 different pack sizes.

Is Rabbit anti-14-3-3 beta Antibody in stock?

Rabbit anti-14-3-3 beta Antibody is currently made to order / not held in immediate stock. Contact us for lead time.

Rabbit anti-14-3-3 beta Antibody

Product Specifications

Background

Rabbit polyclonal antibody to 14-3-3 beta

CAS Number

9007-83-4

Synonyms

14-3-3 protein beta/alpha; Protein 1054; Protein kinase C inhibitor protein 1; KCIP-1

Host

Rabbit

Reactivity

H, M, R

Immunogen

Recombinant full length protein of human 14-3-3 beta

Clonality

Polyclonal

Applications

WB, IHC, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Store at -20°C. Avoid repeated freeze / thaw cycles.

Host or Source

Rabbit

WB Description

Western blot analysis of 14-3-3 beta expression in SW480 (A), HepG2 (B), mouse liver (C), mouse kidney (D) whole cell lysates.

IHC description

Immunohistochemical analysis of 14-3-3 beta staining in rat liver formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunofluorescent analysis of 14-3-3 beta staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.