Anti-Osteocalcin Antibody
Mouse Monoclonal Antibody specific to Bovine Osteocalcin
Product Specifications
Product Name Alternative
Bone Gla protein, BGP, γ-carboxyglutamic acid-containing protein
Gene Name
BGLAP
NCBI Gene ID
<a href="https://www.ncbi.nlm.nih.gov/gene/?term=BGLAP">BGLAP</a>
UniProt
P02818
Cellular Locus
Secreted.
Host
Mouse
Reactivity
Human, Bovine
Immunogen
Bovine osteocalcin
Target Antigen
Osteocalcin
Target
Osteocalcin
Clonality
Monoclonal
Isotype
IgG2a
Type
Antibody
Applications
WB, ELISA, IHC
Field of Research
Hormones/Proteins
Purification Method
Purified by immunoaffinity chromatography
Dilution
Reconstitute lyophilized antibody in 50ul of distilled water (2.0mg/ml final concentration) then dilute further for applications in PBS or media that is identical to that used in the assay system.
Format
Purified
Form
Lyophilized
Buffer
Phosphate Buffered Saline
Function
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Additionnal Information
Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50116.
<br><br>Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.
<br><br>Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.
<br><br>Endusers should determine optimal concentrations for their applications.
Storage Conditions
Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stored.
Product Datasheet
https://www.qedbio.com/wp-content/uploads/pdf/50118.pdf
Specificity
Epitope within residues 45-49; human and bovine.
Formulation
PBS, pH7.4, containing 1.0% bovine serum albumin, lyophlized.
Target Background
Osteocalcin is a noncollagenous, 49 amino acid glutamate-rich polypeptide bone matrix protein with a molecular weight of about 5800Da. Osteoblasts produce osteocalcin and incorporate it into the bone matrix. Osteocalcin is released into the circulation from the matrix during bone resorption and, therefore, is considered a marker of bone turnover rather than a specific marker of bone formation.
CAS Number
9007-83-4
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