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Anti-Lead Antibody

Mouse Monoclonal Antibody specific to Lead

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-Lead Mouse Monoclonal Antibody

Host

Mouse

Reactivity

Lead

Immunogen

Lead conjugated to keyhole limpet hemocyanin (KLH) by proprietary chemistry.

Target

Lead

Clonality

Monoclonal

Isotype

IgM

Type

Antibody

Applications

ELISA

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Whole Antiserum

Additionnal Information

Indirect ELISA: optimized for lead-RSA (rabbit serum albumin) on solid phase at 200ng/well. Ascites diluted 1:100,000 produces an O.D. signal of 0.5. <br><br>Competition ELISA: with lead-RSA on the solid phase at 200ng/well, 50% inhibition of antibody binding is achieved with Pb2+ at 800ng/well. <br><br>These are recommended concentrations. <br><br>End users should determine optimal concentrations for their applications.Not all applications have been investigated.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody detects lead in the ppb range. Indirect ELISA endpoint titer is >1: 100,000.

Formulation

Whole antiserum

Target Background

Exposure to potentially neurotoxic levels of lead occurs in approximately 9% of American children under 6 years of age, and many urban dwellings still contain dangerous levels of lead paint.

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