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Rhod-5N

Rhod-5N is a calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd2+ is higher than other interfering cations ((Na+, K+, Mg2+, Ca2+, Zn2+) except Pb2+), and the detection limit is 3.1 μg/L[1][2].

Product Specifications

CAS Number

888229-31-0

UNSPSC

12171500

Hazard Statement

H302-H315-H319-H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/rhod-5n.html

Solubility

10 mM in DMSO

Smiles

CN(C1=CC2=C(C=C1)C(C(C=C3)=CC(OCCOC(C=C4[N+]([O-])=O)=C(C=C4)N(CC(O[K])=O)CC(O[K])=O)=C3N(CC(O[K])=O)CC([O-])=O)=C(C=CC(N(C)C)=C5)C5=[O+]2)C

Molecular Formula

C39H36K3N5O13

Molecular Weight

900.02

Precautions

P261-P264-P270-P271-P280-P302+P352-P304+P340-P305+P351+P338-P330-P362+P364-P403+P233-P405-P501

References & Citations

[1]Soibinet M, Souchon V, Leray I, et al. Rhod-5N as a fluorescent molecular sensor of cadmium (II) ion[J]. Journal of fluorescence, 2008, 18: 1077-1082.|[2]McNamara C J, Perry Iv T D, Bearce K, et al. Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N[J]. Journal of microbiological methods, 2005, 61 (2) : 245-250.

Shipping Conditions

Room temperature

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Frequently Asked Questions

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