AKR1C3 (Human) ELISA Kit

Aldo-keto reductase 1C3 (AKR1C3) is a member of the aldo-keto reductase protein superfamily. It is primarily expressed in the endocrine organs such as the prostate, adrenal glands, uterus, and breasts, but is also expressed in other organs such as the brain, kidney, liver, and lungs. AKR1C3 catalyzes the formation of androgens such as testosterone and 5α-dihydrotestosterone (5α-DHT). It is also implicated in xenobiotics, estrogen, prostaglandin, and progesterone metabolism. Elevated expression of AKR1C3 is associated with cancers such as head and neck squamous cell carcinoma, breast, esophageal, endometrial, and prostate cancer. Thus, AKR1C3 could serve as a promising biomarker for cancer development or progression. BioVision’s AKR1C3 (Human) ELISA kit is based on the Sandwich ELISA principle to quantitatively measure the concentration of AKR1C3 in human serum, plasma, and other biological fluids. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and then washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the concentration of AKR1C3 in the sample or standard.