Mouse anti Human CD15

Identification of CD15 that recognizes a Human mylelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.1. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils. It is present at low density on circulating monocytes. In lymphoid tissue CD15 reacts with Reed-Sternberg cells of Hodgkins disease and with granulocytes. However, CD15 reacts with only a few tissue macrophages and does not react with dendritic cells.

PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dörken B, Gilks WR, et al, eds. LeucocyteTyping IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989;800-805. 2. Hanjan SNS, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on Human myelomonocytic cells. Clin Immunol Immunopath. 1982;23:172. 3. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984;82:29. 4. Pinkus GS, Thomas P, Said JW. Leu-M1—A marker for Reed Sternberg cells in Hodgkin's Disease: An immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.