Thymidine Phosphorylase, Recombinant from Escherichia coli

<strong>Thymidine Phosphorylase, Recombinant from Escherichia coli</strong>_x000D_ <strong>Catalog number:</strong> B2019685_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 5000 units_x000D_ <strong>Molecular Weight or Concentration:</strong> N/A_x000D_ <strong>Supplied as:</strong> Solution_x000D_ <strong>Applications:</strong> a molecular tool for various biochemical applications_x000D_ <strong>Storage:</strong> 2-8°C_x000D_ <strong>Keywords:</strong> Thymidine:orthophosphate deoxy-D-ribosyltransferase_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Ge C, OuYang L, Ding Q, Ou L. Co-expression of recombinant nucleoside phosphorylase from Escherichia coli and its application Appl Biochem Biotechnol. 2009 Oct;159(1):168-77._x000D_ 2: Gbaj A, Edwards PN, Reigan P, Freeman S, Jaffar M, Douglas KT. Thymidine phosphorylase from Escherichia coli: tight-binding inhibitors as enzyme active-site titrants J Enzyme Inhib Med Chem. 2006 Feb;21(1):69-73._x000D_ 3: Karamitros CS, Somody CM, Agnello G, Rowlinson S. Engineering of the Recombinant Expression and PEGylation Efficiency of the Therapeutic Enzyme Human Thymidine Phosphorylase Front Bioeng Biotechnol. 2021 Dec 17;9:793985._x000D_ 4: Moghaddam A, Bicknell R. Expression of platelet-derived endothelial cell growth factor in Escherichia coli and confirmation of its thymidine phosphorylase activity Biochemistry. 1992 Dec 8;31(48):12141-6._x000D_ 5: Ding Q, Ou L, Wei D, Wei X. Optimum induction of recombinant thymidine phosphorylase and its application Nucleosides Nucleotides Nucleic Acids. 2011 May;30(5):360-8._x000D_ 6: Carlson CA, Stewart GJ, Ingraham JL. Thymidine salvage in Pseudomonas stutzeri and Pseudomonas aeruginosa provided by heterologous expression of Escherichia coli thymidine kinase gene J Bacteriol. 1985 Jul;163(1):291-5._x000D_ 7: Moskaleva ND, Sukhodolets VV. [Escherichia coli K-12 mutants for the thymidine phosphorylase structural gene that retain the anabolic function of the enzyme] Genetika. 1981;17(9):1606-17._x000D_ 8: McNally VA, Gbaj A, Douglas KT, Stratford IJ, Jaffar M, Freeman S, Bryce RA. Identification of a novel class of inhibitor of human and Escherichia coli thymidine phosphorylase by in silico screening Bioorg Med Chem Lett. 2003 Nov 3;13(21):3705-9._x000D_ 9: Esipov RS, Gurevich AI, Chuvikovsky DV, Chupova LA, Muravyova TI, Miroshnikov AI. Overexpression of Escherichia coli genes encoding nucleoside phosphorylases in the pET/Bl21(DE3) system yields active recombinant enzymes Protein Expr Purif. 2002 Feb;24(1):56-60._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/30294618">10: Levene M, Pacitti D, Gasson C, Hall J, Sellos-Moura M, Bax BE. Validation of an Immunoassay for Anti-thymidine Phosphorylase Antibodies in Patients with MNGIE Treated with Enzyme Replacement Therapy Mol Ther Methods Clin Dev. 2018 Aug 28;11:1-8. </a>_x000D_ _x000D_ <strong>Products Related to Thymidine Phosphorylase, Recombinant from Escherichia coli can be found at</strong> <a href="https://moleculardepot.com/product-category/Proteins/"> Proteins</a>