Anti-NPFF1 Antibody

Rabbit Polyclonal Antibody specific to NPFF1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

G-protein coupled receptor 147, RFamide-related peptide receptor OT7T022

Gene Name

NPFFR1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=NPFFR1">NPFFR1</a>

UniProt

Q9GZQ6

Cellular Locus

Cell membrane; Multi-pass membrane protein.

Host

Rabbit

Reactivity

Human

Immunogen

N-terminal sequence MEGEPSQPPNSSWPLS and C-terminal sequence CSHLPLTIPAWDI of human NPFF1.

Target Antigen

Neuropeptide FF receptor 1

Target

NPFF1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by immunoaffinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution <br><br>ELISA: use at 1:1,000-1:5,000 dilution.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human NPFF1 (45 kD).

Formulation

PBS.

Target Background

The central nervous system octapeptide, neuropeptide FF (NPFF), is believed to play a role in pain modulation and opiate tolerance. Two G protein-coupled receptors, NPFF1 and NPFF2, have been isolated from human and rat central nervous system tissues. Localization of mRNA and binding sites of these receptors in the dorsal horn of the spinal cord, the lateral hypothalamus, the spinal trigeminal nuclei, and the thalamic nuclei suggest a role for NPFF in pain modulation.