Human IL-6 Protein, His tag (Animal-Free)

Human IL-6 Protein, His tag (Animal-Free), expressed in E. coli

Product Specifications

Background

Interleukin-6 (IL-6) is a pleiotropic, 22-28 kDa cytokine which plays fundamental role in the acute phase response, inflammation, bone metabolism, lymphocyte differentiation and cancer progression. Deregulation of IL-6 production was also found in several diseases, including rheumatoid arthritis, Alzheimer’s disease, autoimmune deficiency disease and different types of cancer.

Product Name Alternative

IFN-β2, B-Cell Differentiation Factor (BCDF), BSF-2, HPGF, HSF, MGI-2

Accession Number

P05231

Tag

IL-6

Type

Cytokines and proteins

Applications

His tag

Purity

> 98% as determined by SDS-PAGE.

Activity

Measure by its ability to induce proliferation in TF-1 cells. The ED₅₀ for this effect is <0.5 ng/mL.The specific activity of recombinant human IL-6 is approximately >1 x 10⁸ IU/mg.

Molecular Weight

21.8 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

The product is shipped with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human IL-6 Protein consists of 183 amino acids and predicts a molecular mass of 21.8 kDa

Formulation

Lyophilized from sterile PBS, pH 8.0

Applications Notes

Human

Other Product Names

Human IL-6 Protein, His tag (Animal-Free), IL-6, IFN-β2, B-Cell Differentiation Factor (BCDF), BSF-2, HPGF, HSF, MGI-2

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IL-6 Protein, His tag (Animal-Free) using the buffer we provided not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.