Anti-BDNF Antibody

Rabbit Polyclonal Antibody specific to BDNF

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

BDNF, Abrineurin [Cleaved into: BDNF precursor form

Gene Name

BDNF

NCBI Gene ID

UniProt

P23560

Cellular Locus

Secreted

Host

Rabbit

Reactivity

Human, Mouse

Immunogen

Synthetic peptide corresponding to the N-terminus of human BDNF.

Target Antigen

Brain-derived neurotrophic factor

Target

BDNF

Clonality

Polyclonal

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Important signaling molecule that activates signaling cascades downstream of NTRK2 (PubMed:11152678). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. {PubMed:11152678, PubMed:12553913, PubMed:29909994}.; [BDNF precursor form]: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2 (PubMed:24908487, PubMed:29909994). Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse (By similarity). {UniProtKB:P21237, PubMed:24908487, PubMed:29909994}.

Additionnal Information

Immunoblotting: use at dilution of 1:1,000. A band of ~27-28kDa is detected. Immunofluorescence: use at dilution of 1:100 These are recommended working dilutions. Endusers should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human and mouse BDNF.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

BDNF acts on neurons of the central nervous system and the peripheral nervous system supporting the survival of existing neurons and stimulating growth and differentiation of new neurons and synapses. In the brain, it is active in the hippocampus, cortex, and basal forebrain, areas vital to learning, memory, and higher thinking. It is also expressed in the retina, motor neurons, kidneys, saliva, and prostate.