Human CD28H / IGPR-1 Protein, His Tag

CD28 homolog (CD28H), also called transmembrane and immunoglobulin domain containing 2 (TMIGD2) and IGPR-1, is encoded by the TMIGD2 gene. CD28H is constitutively expressed on naive T and NK cells. After interaction of CD28 homolog with B7 homologue, the peripheral effector and memory T cells can be activated and proliferated by Akt-dependent signalling cascade. Plays a role in cell-cell interaction, cell migration, and angiogenesis. Through interaction with HHLA2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.9 kDa. The protein migrates as 22-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

Q96BF3-1

Expression Region

Leu 23 - Gly 150

Host

HEK293

Target

CD28H

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human CD28H, His Tag (Cat. No. CDH-H52H3) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-H7, Fc Tag, Avi Tag (Cat. No. B77-H82F5) with a linear range of 10-156 ng/mL (QC tested).