Recombinant Human CCL19, biotinylated

Recombinant human CCL19 produced in E. coli

Product Specifications

CAS Number

9000-83-3

Product Name Alternative

rHuCCL19-biotin, MIP-3beta

Gene Name

CCL19

UniProt

Q99731

Cellular Locus

Secreted.

Reactivity

Human

Target Antigen

C-C motif chemokine 19

Target

CCL19ylated

Type

Protein

Applications

Migration assay

Dilution

Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.

Form

Lyophilized

Function

May play a role not only in inflammatory and immunological responses but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to sPubMed:9498785}.

Additionnal Information

Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration Assay with cells expressing recombinant CCR7.

Storage Conditions

12 months from date of receipt, -20°C to -70°C, as supplied. 1 month,- 20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution. Avoid multiple freeze-thaw cycles.

Specificity

CCL19ylated

Target Background

CCL19 (also known as Macrophage Inflammatory Protein-3beta, MIP-3b, and EBI1 ligand chemokine, ELC) directs chemotaxis of dendritic cells and certain B- and T-lymphocytes, but not monocytes or granulocytes. It is constitutively expressed in thymus and lymph nodes and binds specifically to target cells expressing the receptor CCR7. As a homeostatic chemokine, its primary physiological role is thought to be in the normal recirculation and homing of lymphocytes. However, CCL19 can also be proinflammatory and is implicated in post-HIV infection responses.