Cas9 Protein (High Purity Enzyme)

<strong>Cas9 Protein (High Purity)</strong>_x000D_ <strong>Catalog number:</strong> B2010495_x000D_ <strong>Lot number:</strong> Batch Dependent_x000D_ <strong>Expiration Date:</strong> Batch dependent_x000D_ <strong>Amount:</strong> 50 ug_x000D_ <strong>Molecular Weight or Concentration:</strong> 160 KD_x000D_ <strong>Supplied as:</strong> Lyophilized powder_x000D_ <strong>Applications:</strong> a tool in functional genomics and genome editing as well as other genetic and biochemical applications and uses._x000D_ <strong>Storage:</strong> −20°C_x000D_ <strong>Keywords:</strong> SpCas9, SpyCas9, Cas9, cas5, csn1_x000D_ <strong>Grade:</strong> Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ <strong>References:</strong>_x000D_ 1: Mehta A, Merkel OM. Immunogenicity of Cas9 Protein. J Pharm Sci. 2020_x000D_ Jan;109(1):62-67._x000D_ _x000D_ 2: Bondy-Denomy J. Protein Inhibitors of CRISPR-Cas9. ACS Chem Biol. 2018 Feb_x000D_ 16;13(2):417-423._x000D_ _x000D_ 3: Carmignotto GP, Azzoni AR. On the expression of recombinant Cas_9 protein in_x000D_ E. coli BL21(DE3) and BL21(DE3) Rosetta strains. J Biotechnol. 2019 Dec_x000D_ 20;306:62-70._x000D_ _x000D_ 4: Laustsen A, Bak RO. Electroporation-Based CRISPR/Cas_9 Gene Editing Using Cas9_x000D_ Protein and Chemically Modified sgRNAs. Methods Mol Biol. 2019;1961:127-134._x000D_ _x000D_ 5: Xiao Q, Guo D, Chen S. Application of CRISPR/Cas_9-Based Gene Editing in_x000D_ HIV-1/AIDS Therapy. Front Cell Infect Microbiol. 2019 Mar 22;9:69._x000D_ _x000D_ 6: Khadempar S, Familghadakchi S, Motlagh RA, Farahani N, Dashtiahangar M,_x000D_ Rezaei H, Gheibi Hayat SM. CRISPR-Cas_9 in genome editing: Its function and_x000D_ medical applications. J Cell Physiol. 2019 May;234(5):5751-5761._x000D_ _x000D_ 7: Suresh B, Ramakrishna S, Kim H. Cell-Penetrating Peptide-Mediated Delivery of_x000D_ Cas_9 Protein and Guide RNA for Genome Editing. Methods Mol Biol._x000D_ 2017;1507:81-94._x000D_ _x000D_ 8: Liu W, Yang C, Liu Y, Jiang G. CRISPR/Cas_9 System and its Research Progress_x000D_ in Gene Therapy. Anticancer Agents Med Chem. 2019;19(16):1912-1919._x000D_ _x000D_ 9: Li L, Hu S, Chen X. Non-viral delivery systems for CRISPR/Cas9-based genome_x000D_ editing: Challenges and opportunities. Biomaterials. 2018 Jul;171:207-218._x000D_ <a href="https://pubmed.ncbi.nlm.nih.gov/32217751/">10: Walton RT, Christie KA, Whittaker MN, Kleinstiver BP. Unconstrained genome_x000D_ targeting with near-PAMless engineered CRISPR-Cas9 variants. Science. 2020 Apr_x000D_ 17;368(6488):290-296.</a>_x000D_ _x000D_ <strong>Products Related to Cas9 Protein (High Purity)</strong> <a href="https://moleculardepot.com/product-category/Proteins/">: Proteins</a>