Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)

Biotinylated SARS-CoV-2 S1 protein, His,Avitag (S1N-C82E9) is expressed from human 293 cells (HEK293).

Product Specifications

Background

It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

Specifications

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 78.6 kDa. The protein migrates as 100-150 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Host

HEK 293

Target

Spike S1

Conjugation

Biotin-labeled

Tag

C-10×His & C-Avi

Source

SARS-CoV-2

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 2 μg/mL (100 μL/well) can bind Biotinylated SARS-CoV-2 S1 protein, His,Avitag (Cat. No. S1N-C82E9) with a linear range of 20-312 ng/mL (QC tested).