Anti-STAT6 (phospho-Thr645) Antibody

Rabbit Polyclonal Antibody specific to STAT6 (phospho-Thr645)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-STAT6 (phospho-Thr645) Rabbit Polyclonal Antibody

Gene Name

STAT6

Gene ID

601512

NCBI Gene ID

UniProt

P42226

Cellular Locus

Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation.

Host

Rabbit

Reactivity

Human

Immunogen

Peptide sequence that includes phosphorylation site of threonine 645 (P-A-T(p)-I-K) derived from human STAT6 and conjugated to KLH.

Target Antigen

Signal transducer and activator of transcription 6

Target

STAT6 (phospho-Thr645)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IHC

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Carries out a dual signal transduction and activation of transcription. Involved in IL4/interleukin-4- and IL3/interleukin-3-mediated signaling. {PubMed:17210636}.

Additionnal Information

Immunoblotting: use at dilution of 1:500-1:1,000. A band of~110kDa is detected. Immunohistochemistry: use at dilution of 1:50- 1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human STAT6 only when phosphorylated at threonine 645.

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Signal transducers and activators of transcription (STATs) are transcription factors that play important roles in signaling pathways induced by many different cytokines and growth factors that regulate cell growth and differentiation, the immune response, antiviral activity, and homeostasis. Their activation and mechanisms of action are examples of direct signaling to the nucleus by extracellular polypeptide ligands without the involvement of second messengers, tyrosine phosphorylation of transcription factors, or transcription factors that make direct contact with membrane-bound receptors and DNA. Since the discovery of STAT 1 and 2, the family has been expanded to seven members.