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DOTAP (chloride)

DOTAP chloride is a useful and effective cationic lipid for transient and stable transfection DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides) with out the use of helper lipid[1].

Product Specifications

CAS Number

132172-61-3

Product Name Alternative

1,2-Dioleoyl-3-trimethylammonium-propane (chloride)

UNSPSC

12352211

Hazard Statement

H302, H315, H319, H335

Target

Liposome

Type

Oligonucleotides

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Cancer

Assay Protocol

https://www.medchemexpress.com/dotap-chloride.html

Purity

99.91

Solubility

DMSO : 25 mg/mL (ultrasonic; warming; heat to 60°C) |Ethanol : 130 mg/mL (ultrasonic)

Smiles

CCCCCCCC/C=C\CCCCCCCC(OCC(OC(CCCCCCC/C=C\CCCCCCCC)=O)C[N+](C)(C)C)=O.[Cl-]

Molecular Formula

C42H80ClNO4

Molecular Weight

698.54

Precautions

H302, H315, H319, H335

References & Citations

[1]Kim BK, et al. DOTAP/DOPE ratio and cell type determine transfection efficiency with DOTAP-liposomes.Biochim Biophys Acta. 2015 Oct;1848 (10 Pt A) :1996-2001.

Shipping Conditions

Blue Ice

Storage Conditions

-20°C (Powder, protect from light, stored under nitrogen)

Scientific Category

Oligonucleotides

Clinical Information

Phase 2

Citation 01

Adv Healthc Mater. 2023 Jan;12 (6) :e2202412.|bioRxiv. 2025 Oct 1.|Exploration. 2024 Jun 26;5 (1) :20240002.|Microbiol Spectr. 2025 Nov 5;13 (12) :e0143825.|Nanoscale. 2023 Nov 16;15 (44) :17825-17838.|Nat Immunol. 2025 Oct;26 (10) :1660-1672.|Pharmaceutics. 2023 Apr 30;15 (5) :1379.|SSRN. 2024 Nov 12.|University of Washington. 2025.|Drug Dev Ind Pharm. 2024 Aug 7:1-16.|Signal Transduct Target Ther. 2025 Mar 24;10 (1) :101.

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