Anti-DEDAF Antibody

Rabbit Polyclonal Antibody specific to DEDAF

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Apoptin-associating protein 1, APAP-1, Death effector domain-associated factor, DED-associated factor, YY1 and E4TF1-associated factor 1

Gene Name

RYBP

Gene ID

23429

NCBI Gene ID

UniProt

Q8N488

Accession Number

NP_036366

Cellular Locus

Nucleus, Cytoplasm, Nucleus, nucleoplasm

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 215-229 of human DEDAF (accession no. AF179286). This sequence is identical to that of mouse.

Target Antigen

RING1 and YY1-binding protein

Target

DEDAF

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IHC-P, IF

Field of Research

Apoptosis

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1-like complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed:25519132). Component of a PRC1-like complex that mediates monoubiquitination of histone H2A 'Lys-119' on the X chromosome and is required for normal silencing of one copy of the X chromosome in XX females. May stimulate ubiquitination of histone H2A 'Lys-119' by recruiting the complex to target sites (By similarity). Inhibits ubiquitination and subsequent degradation of TP53, and thereby plays a role in regulating transcription of TP53 target genes (PubMed:19098711). May also regulate the ubiquitin-mediated proteasomal degradation of other proteins like FANK1 to regulate apoptosis (PubMed:14765135, PubMed:27060496). May be implicated in the regulation of the transcription as a repressor of the transcriptional activity of E4TF1 (PubMed:11953439). May bind to DNA (By similarity). May play a role in the repression of tumor growth and metastasis in breast cancer by down-regulating SRRM3 (PubMed:27748911). {UniProtKB:Q8CCI5, PubMed:11953439, PubMed:14765135, PubMed:19098711, PubMed:27060496, PubMed:27748911}.

Additionnal Information

Immunoblotting: use at 1ug/mL. Immunohistochemistry: use at 10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Whole cell lysate from A549 or HepG2 cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat DEDAF (32kDa).

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. A novel protein that interacts with the death effector domain of capase-8 and caspase-10 has been designated DEDAF and is dedentical to the transcriptional repressor RYBP. DEDAF is expressed in multiple tissues and cell lines and appears to be involved in the regulation of both cytoplasmic and nuclear events of apoptosis.