Tilmicosin ELISA Kit

Tilmicosin is a semi-synthetic macrolide antibiotic that is structurally similar to Tylosin. As a macrolide, its main function is to bind to 23s rRNA of the 50S ribosomal subunit of the bacteria. In doing so, it prevents the transpeptidation/translocation step and thereby inhibits bacterial protein synthesis. It targets both gram-positive and gram-negative bacteria that cause respiratory infection in cattle and pigs. BioVision’s Tilmicosin ELISA kit is used to quantitatively measure Tilmicosin in muscle and liver tissue, honey, milk, and egg samples. The kit is based on the Competitive ELISA principle. Samples and standards are added to the microwell plate that is pre-coated with an antigen and competes for binding to the anti-Tilmicosin antibody. The HRP conjugate is added to each well and any unattached conjugates are washed off using Wash Buffer. The HRP enzymatic reaction is detected by the addition of substrate reagents. Finally, the reaction is terminated with an acidic stop solution. The color developed is inversely proportional to the concentration of Tilmicosin in the samples.