Goat Anti-Mouse/Rabbit IgG (H&L) - Polymer Antibody (HRP)

The Goat Mouse/Rabbit IgG (H&L) -HRP Polymer Antibody is suitable for ELISA, ICC, IHC, WB. It is a Polyclonal, HRP conjugated antibody which raised against Mouse/Rabbit IgG. Purification: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.