Recombinant TOP2A Antibody / Topoisomerase II alpha
It recognizes a 170kDa protein, which is identified as topoisomerase IIAlpha. It shows no cross-reaction with Topoisomerase II beta or I. Topo II Alpha plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is reported to be a sensitive and specific marker of late S-, G2- & M-phases in transformed and developmentally regulated normal cells. Topo II Alpha is also implicated in drug resistance of tumor cells and has been shown to be over-expressed in many human cancers. Decreased expression of Topo IIa is the predominant mechanism of resistance to several chemotherapeutic agents.
Product Specifications
UniProt
P11388
Reactivity
Human
Immunogen
A recombinant human Topoisomerase II alpha protein fragment (aa1300-1500) was used as the immunogen for the recombinant TOP2A antibody.
Clonality
Recombinant
Clone
RTOP2A/8339
Conjugation
Unconjugated
Field of Research
Signal Transduction
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 0.5-1ug/ml for 30 minutes at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant TOP2A antibody should be determined by the researcher.
Tested Applications
IHC-P
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG1, kappa
Available Sizes
Frequently Asked Questions
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