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Glial Fibrillary Acidic Protein Antibody / GFAP

This mAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP) . It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system.Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

Product Specifications

UniProt

P14136

Reactivity

Human

Immunogen

A recombinant partial protein sequence (within amino acids 150-250) from the human protein was used as the immunogen for the Glial Fibrillary Acidic Protein antibody.

Clonality

Monoclonal

Clone

GFAP/6876

Conjugation

Unconjugated

Field of Research

Neuroscience

Purification

Protein A/G affinity

Dilution

Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT, Western blot: 2-4ug/ml

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Optimal dilution of the Glial Fibrillary Acidic Protein antibody should be determined by the researcher.

Tested Applications

IHC-P, WB

Host or Source

Mouse

Preservative

1 mg/ml in 1X PBS; rAlbumin free, sodium azide free

Isotype

Mouse IgG1, kappa

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