GFAP Antibody / Glial Fibrillary Acidic Protein
GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.
Product Specifications
UniProt
P14136
Reactivity
Human
Immunogen
A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the GFAP antibody.
Clonality
Recombinant
Clone
GFAP/8616R
Conjugation
Unconjugated
Field of Research
Neuroscience
Purification
Protein A/G affinity
Dilution
Immunohistochemistry (FFPE) : 1-2ug/ml for 30 minutes at RT, Western blot: 2-4ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the GFAP antibody should be determined by the researcher.
Tested Applications
IHC-P, WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa
Frequently Asked Questions
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