Recombinant Bax Antibody
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Product Specifications
UniProt
Q07812
Reactivity
Human
Immunogen
A portion of amino acids 1-100 from human BAX protein was used as the immunogen for the recombinant Bax antibody.
Clonality
Recombinant
Clone
BAX/8960R
Conjugation
Unconjugated
Purification
Protein A/G affinity
Dilution
Western blot: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the recombinant Bax antibody should be determined by the researcher.
Tested Applications
WB
Host or Source
Rabbit
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Rabbit IgG, kappa
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