SDHB Antibody / Succinate dehydrogenase B
Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation. Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.
Product Specifications
UniProt
P21912
Reactivity
Human
Immunogen
A recombinant human SDHB protein fragment (within amino acids 165-273) was used as the immunogen for the SDHB antibody.
Clonality
Monoclonal
Clone
SDHB/3745
Conjugation
Unconjugated
Field of Research
Metabolism Research
Purification
Protein A/G affinity
Dilution
Immunofluorescence: 1-2ug/ml, Immunohistochemistry (FFPE) : 1-2ug/ml for 30 min at RT
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the SDHB antibody should be determined by the researcher.
Tested Applications
IF, IHC-P
Host or Source
Mouse
Preservative
1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
Isotype
Mouse IgG2, kappa
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