Anti-NIK (CT) Antibody

Rabbit Polyclonal Antibody specific to NIK (CT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.11.25, NF-kappa-beta-inducing kinase, HsNIK, Serine/threonine-protein kinase NIK

Gene Name

MAP3K14

Gene ID

9020

NCBI Gene ID

UniProt

Q99558

Accession Number

NP_003945.2

Cellular Locus

Cytoplasm.

Host

Rabbit

Reactivity

Human

Immunogen

Synthetic peptide corresponding to aa 931-947 of human NIK (accession no. Q99558).

Target Antigen

Mitogen-activated protein kinase kinase kinase 14

Target

NIK (CT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, IF, ICC

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Lymphotoxin beta-activated kinase which seems to be exclusively involved in the activation of NF-kappa-B and its transcriptional activity. Promotes proteolytic processing of NFKB2/P100, which leads to activation of NF-kappa-B via the non-canonical pathway. Could act in a receptor-selective manner. {PubMed:15084608}.

Additionnal Information

Immunoblotting: Recommended concentration: 2-4ug/ml. <br><br>Optimal dilution should be determined by End user. <br><br>Positive control: Whole cell lysate of NIK-transfected 293 cells.

Storage Conditions

This antibody is stable for 3 months at 4°C and at least 1 year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

This antibody recognizes human NIK.

Formulation

PBS, 1mg/ml, pH 7.4, 0.02% sodium azide.

Buffer pH

pH 7.4

Target Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha, LPS, and mitogens. A serine/threonine protein kinase which mediates NF-kappaB activation by IL-1, TNFalpha, and CD95 has been identified and designated NIK (for NF-kappaB inducing kinase). NIK is an activator of IkappaB kinase alpha and beta (IKKalpha and IKKbeta). Therefore, NIK is a key molecule in the NF-kappaB signaling pathway leading to the expression of a variety of gene products in response to pro-inflammatory cytokines and bacterial products.