HLA-ABC Antibody
Reacts with a monomorphic determinant of human major histocompatibility (MHC) class I antigens (HLA-A, B and C) . Immunoprecipitation of lactoperoxidase radioiodinated cell surface proteins and surface sialoglycoproteins radiolabeled by sodium metaperiodate/tritiated sodium borohydride technique confirmed the structure gp44, p12 (consisting of a 44kDa glycopeptide linked with a non-glycosylated 12kDa peptide) typical for MHC class I antigen (s) as a structure recognized by Bra23/9. Human MHC class I antigens are expressed constitutively on all nucleated cells lymphocytes such as lymphocytes, thymocytes, granulocytes, and bone marrow cells and are absent on erythrocytes. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation.
Product Specifications
UniProt
P30443, P30499, P01889
Reactivity
Human
Immunogen
Human REH cells (a pre-B leukaemia cell line) was used as the immunogen for the HLA-ABC antibody.
Clonality
Monoclonal
Clone
Bra-23/9
Conjugation
Unconjugated
Field of Research
Immunology & Inflammation; Cell Biology
Purification
Protein A/G affinity
Dilution
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2ug/ml
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Optimal dilution of the HLA-ABC antibody should be determined by the researcher.
Tested Applications
FACS, IF
Host or Source
Mouse
Preservative
0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin, 0.05% sodium azide
Isotype
Mouse IgG2a, kappa
Available Sizes
Frequently Asked Questions
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