RFP Fusion Human Mesothelin / MSLN (296-580) Protein, His Tag

RFP Fusion Human Mesothelin (296-580), His Tag (MSN-HR2P4) is expressed from human 293 cells (HEK293) . It contains AA Glu 296-Gly 580 (Accession # AAH09272.1) .

Product Specifications

Background

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 62 kDa. The protein migrates as 66-80 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH09272.1

Expression Region

Glu 296-Gly 580

Host

HEK293

Target

Mesothelin

Conjugation

RFP Fusion

Tag

C-10xHis

Source

Human

Stability

● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

95%

Bioactivity

Immobilized Anti-Human MSLN Mab, Human IgG1 at 10 μg/mL (100 μL/well) can bind RFP Fusion Human Mesothelin (296-580), His Tag (Cat. No. MSN-HR2P4) with a linear range of 0.078-0.625 μg/mL (QC tested).