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HSF1 Antibody

Rat Anti-Mouse HSF1 Monoclonal IgG1

Product Specifications

Product Name Alternative

HSF1, Heat shock factor protein 1, Heat shock transcription factor 1, HSTF1, HSF 1

UniProt

P38532

Reactivity

Bovine, Guinea pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat

Immunogen

Purified recombinant mouse HSF1 protein, with epitope mapping to amino acids 378-395

Target

HSF1

Clonality

Monoclonal

Clone

10H8

Conjugation

Unconjugated

Field of Research

Epigenetics & Chromatin

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), IHC (1:1000), ICC/IF (1:200) ; optimal dilutions for assays should be determined by the user.

Molecular Weight

95kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of HSF1 in 20 μg of heat shocked HeLa cell lysate by ECL immunoblot analysis using Goat anti-rat IgG: HRP as the secondary antibody

Tested Applications

ELISA, Enzyme Assay, ICC, IF, IHC, IP, WB

NCBI Accession Number

NP_032322.1

Host or Source

Rat

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG1

Entrez

15499

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