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Nitrotyrosine Antibody

Mouse Anti- Nitrotyrosine Monoclonal IgG2a

Product Specifications

Product Name Alternative

Nitrotyrosine, Nitro tyrosine, 3-Nitrotyrosine

Reactivity

All

Immunogen

3- (4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Target

Nitrotyrosine

Clonality

Monoclonal

Clone

39B6

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Neuroscience, Protein Biochemistry, Signal Transduction

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1400), IHC (1:100) ; optimal dilutions for assays should be determined by the user.

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

0.7 μg/ml was sufficient for detection of 5 μg SIN-1 treated BSA by Western Blot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

AM, ELISA, FC, ICC, IF, IHC, IP, WB

Host or Source

Mouse

Preservative

PBS, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG2a

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