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HSP27 Antibody

Mouse Anti-Human HSP27 Monoclonal IgG2b Kappa

Product Specifications

Product Name Alternative

HSPB1, HSP27, HSP25, HSP28, Heat shock protein beta-1, Heat shock 27 kDa protein, MKBP, DMPK-binding protein, SRP27, 28kDa heat shock protein, CMT2F

UniProt

P04792

Reactivity

Human

Immunogen

Full length human HSP27

Target

HSP27

Clonality

Monoclonal

Clone

5D12-A12

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cardiovascular Research, Cell Biology, Protein Biochemistry, Signal Transduction

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:2000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Molecular Weight

27kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

0.5 μg/ml was sufficient for detection of HSP27 in 10 μg of HeLa lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ELISA, FC, ICC, IF, IHC, IP, WB

NCBI Accession Number

NP_001531.1

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG2b Kappa

Entrez

3315

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