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HSP70 Antibody

Mouse Anti-Human HSP70 Monoclonal IgG1

Product Specifications

Product Name Alternative

HSPA1A, HSPA1B, HSPA1, HSP70, HSP70-1, HSP70.1, HSP70-2, HSP72, HSP73, HSX70, Heat shock 70 kDa protein 1A, Heat shock 70 kDa protein 1B

UniProt

P0DMV8, P0DMV9

Reactivity

Amphibian, Drosophila, Fish, Gallus, Human, Mouse, Rat, Saccharomyces, Yeast

Immunogen

Human recombinant HSP70 overexpressed in E.coli

Target

HSP70

Clonality

Monoclonal

Clone

5A5

Conjugation

Unconjugated

Field of Research

Cancer Biology, Cell Biology, Protein Biochemistry, Signal Transduction

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:500), IP (1μg) ; optimal dilutions for assays should be determined by the user.

Molecular Weight

70kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of HSP70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

AM, ICC, IF, IHC, IP, WB

NCBI Accession Number

NP_005336.3

Host or Source

Mouse

Preservative

PBS pH 7.2, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG1

Entrez

3303

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