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HDEL Antibody

Mouse Anti-Yeast HDEL Monoclonal IgG2b

Product Specifications

Product Name Alternative

H-D-E-L (his-asp-glu-leu), endoplasmic reticulum, luminal ER protein retention, KDELR1, Endoplasmic reticulum retention signal

Reactivity

Drosophila, Plant, Saccharomyces, Yeast

Immunogen

Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip

Target

HDEL

Clonality

Monoclonal

Clone

2E7

Conjugation

Unconjugated

Field of Research

Cell Biology

Purification

Protein G Purified

Concentration

1 mg/ml

Dilution

WB (1:1000), ICC/IF (1:100) ; optimal dilutions for assays should be determined by the user.

Molecular Weight

78kDa

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

1 μg/ml was sufficient for detection of HDEL-containing proteins in 10 μg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

Tested Applications

ICC, IF, WB

Host or Source

Mouse

Preservative

PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.

Isotype

IgG2b

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