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Anti-PKD/PKCμ (phospho-Ser738) Antibody

Rabbit Polyclonal Antibody specific to PKD/PKCμ (phospho-Ser738)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-PKD/PKCμ (phospho-Ser738) Rabbit Polyclonal Antibody

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide sequence that includes phosphorylation site of Serine 738 (E-K-S(p)-F-R) derived from human PKD/PCKu and conjugated to KLH.

Target

PKD/PKCμ (phospho-Ser738)

Clonality

Polyclonal

Type

Antibody

Applications

WB, IF

Field of Research

Phosphospecific Antibodies

Purification Method

Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Additionnal Information

Immunoblotting: use at dilution of 1:500- 1:1,000. A band of ~115kDa is detected. <br><br>Immunofluorescence: use at dilution of 1:100- 1:200. <br><br>These are recommended working dilutions. <br><br>End users should determine optimal dilutions for their applications.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody detects endogenous human, mouse, and rat PKD/PKCu only when phosphorylated at serine 738.Accession no.: Q15139, NP_002733.2

Formulation

PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Buffer pH

pH 7.4

Target Background

Protein kinase C (PKC), a family of lipid-activated serine kinases, is involved in multiple functions in the regulation of growth control. The PKC-related isoform PKC mu/PKD has been implicated in mitogenic signal cascades because of the activation of p42/p44 MAPK leading to Elk1-mediated gene transcription, and PKC mu/PKD has been shown to be activated via a PKC-dependent pathway.

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