Mouse anti-Actin Sarcomeric Muscle Antibody
Product Specifications
Background
Mouse monoclonal antibody to Actin Sarcomeric Muscle
CAS Number
9007-83-4
Synonyms
ACTA; Actin alpha skeletal muscle; Alpha-actin-1; ACTC; Actin alpha cardiac muscle 1; Alpha-cardiac actin
Host
Mouse
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human Actin Sarcomeric Muscle. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/300)
Buffer
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
IHC description
Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human skeletal muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IP description
Immunohistochemical analysis of Actin Sarcomeric Muscle staining in human myocardium formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
58; 70
SwissProt (Human)
P68133; P68032
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