Mouse anti-Topoisomerase 2 alpha Antibody
Product Specifications
Background
Mouse monoclonal antibody to Topoisomerase 2 alpha
CAS Number
9007-83-4
Synonyms
TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II alpha isozyme
Host
Mouse
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IH, IF
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)
Buffer
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates.
IHC description
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IP description
Immunohistochemical analysis of Topoisomerase 2 alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
7153
SwissProt (Human)
P11388
Available Sizes
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC402853-100 1x 100 µL
CD54 / ICAM-1 (15.2), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC402823-100 1x 100 µL
BMI1 (Oncogene and Stem Cell Marker) (BMI1/2823), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details