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Mouse anti-Topoisomerase 2 alpha Antibody

Selected Variant

DL99645A-01

Size: 50 µL

Product Specifications

Background

Mouse monoclonal antibody to Topoisomerase 2 alpha

CAS Number

9007-83-4

Synonyms

TOP2; DNA topoisomerase 2-alpha; DNA topoisomerase II alpha isozyme

Host

Mouse

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human Topoisomerase 2 alpha. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IH, IF

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)

Buffer

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of Topoisomerase 2 alpha expression in Hela (A), HepG2 (B), K562 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunohistochemical analysis of Topoisomerase 2 alpha staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

7153

SwissProt (Human)

P11388

Available Sizes

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