Mouse anti-Ki67 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Ki67

CAS Number

9007-83-4

Synonyms

Antigen KI-67

Host

Mouse

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human Ki67. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

IH, IF

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

IH (1/100 - 1/300), IF (1/100 - 1/500)

Buffer

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

IHC description

Immunohistochemical analysis of Ki67 staining in human classical hodgkin's lymphoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunohistochemical analysis of Ki67 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.