Mouse anti-Claudin 3 Antibody

Product Specifications

Background

Mouse monoclonal antibody to Claudin 3

CAS Number

9007-83-4

Synonyms

C7orf1; CPETR2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1

Host

Mouse

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human Claudin 3. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/300)

Buffer

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of Claudin 3 expression in MCF7 (A) whole cell lysates.

IHC description

Immunohistochemical analysis of Claudin 3 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IF/ICC description

Immunohistochemical analysis of Claudin 3 staining in human intestine formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

IP description

Immunohistochemical analysis of Claudin 3 staining in human rectum formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.