Mouse anti-Cytokeratin 14 Antibody
Product Specifications
Background
Mouse monoclonal antibody to Cytokeratin 14
CAS Number
9007-83-4
Synonyms
Keratin type I cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14
Host
Mouse
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 14. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/300)
Buffer
Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of Cytokeratin 14 expression in A431 (A) whole cell lysates.
IHC description
Immunohistochemical analysis of Cytokeratin 14 staining in human esophageal squamous carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunohistochemical analysis of Cytokeratin 14 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IP description
Immunohistochemical analysis of Cytokeratin 14 staining in human squamous cell lung carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
3861
SwissProt (Human)
P02533
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