Mouse anti-MLH1 Antibody
Product Specifications
Background
Mouse monoclonal antibody to MLH1
CAS Number
9007-83-4
Synonyms
COCA2; DNA mismatch repair protein Mlh1; MutL protein homolog 1
Host
Mouse
Reactivity
H
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within human MLH1. The exact sequence is proprietary.
Clonality
Monoclonal
Applications
WB, IH, IF
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/100 - 1/300), IF (1/100 - 1/500)
Buffer
Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of MLH1 expression in K562 (A), HEK293 (B), Hela (C) whole cell lysates.
IHC description
Immunohistochemical analysis of MLH1 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunohistochemical analysis of MLH1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
4292
SwissProt (Human)
P40692
Available Sizes
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