Mouse anti-PCNA Antibody

Product Specifications

Background

Mouse monoclonal antibody to PCNA

CAS Number

9007-83-4

Synonyms

Proliferating cell nuclear antigen; PCNA; Cyclin

Host

Mouse

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within human PCNA. The exact sequence is proprietary.

Clonality

Monoclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/100 - 1/300)

Buffer

Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of PCNA expression in K562 (A), HEK293 (B), A431 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of PCNA staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.