Rabbit anti-GPR92 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to GPR92
CAS Number
9007-83-4
Synonyms
GPR92; GPR93; Lysophosphatidic acid receptor 5; LPA receptor 5; LPA-5; G-protein coupled receptor 92; G-protein coupled receptor 93
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR92. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of GPR92 expression in HuT78 (A), H1792 (B), A375 (C), mouse spleen (D), mouse heart (E), rat spleen (F), rat heart (G) whole cell lysates.
IHC description
Immunohistochemical analysis of GPR92 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of GPR92 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
57121
SwissProt (Human)
Q9H1C0
SwissProt (Mouse)
Q149R9
Available Sizes
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