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Rabbit anti-UPF1 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to UPF1

CAS Number

9007-83-4

Synonyms

KIAA0221; RENT1; Regulator of nonsense transcripts 1; ATP-dependent helicase RENT1; Nonsense mRNA reducing factor 1; NORF1; Up-frameshift suppressor 1 homolog; hUpf1

Host

Rabbit

Reactivity

H, M

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human UPF1. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of UPF1 expression in CT26 (A), PC3 (B), MCF7 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of UPF1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Gene ID (Human)

5976

Gene ID (Mouse)

19704

SwissProt (Human)

Q92900

SwissProt (Mouse)

Q9EPU0

Available Sizes

Frequently Asked Questions

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