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Rabbit anti-5-HT5A Antibody

Product Specifications

Background

Rabbit polyclonal antibody to 5-HT5A

CAS Number

9007-83-4

Synonyms

5-hydroxytryptamine receptor 5A; 5-HT-5; 5-HT-5A; 5-HT5A; Serotonin receptor 5A

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human 5-HT5A. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of 5-HT5A expression in mouse liver (A), mouse kidney (B), rat liver (C), rat kidney (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of 5-HT5A staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

3361

Gene ID (Mouse)

15563

Gene ID (Rat)

25689

SwissProt (Human)

P47898

SwissProt (Mouse)

P30966

SwissProt (Rat)

P35364

Available Sizes

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