Rabbit anti-DNA Polymerase lambda Antibody

Product Specifications

Background

Rabbit polyclonal antibody to DNA Polymerase lambda

CAS Number

9007-83-4

Synonyms

DNA polymerase lambda; Pol Lambda; DNA polymerase beta-2; Pol beta2; DNA polymerase kappa

Host

Rabbit

Reactivity

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human DNA Polymerase lambda. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IH

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IH (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of DNA Polymerase lambda expression in HepG2 (A), SGC7901 (B), Panc1 (C) whole cell lysates.

IHC description

Immunohistochemical analysis of DNA Polymerase lambda staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.