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Rabbit anti-PRDM12 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to PRDM12

CAS Number

9007-83-4

Synonyms

PFM9; PR domain zinc finger protein 12; PR domain-containing protein 12

Host

Rabbit

Reactivity

H

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PRDM12. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of PRDM12 expression in U87MG (A), MCF7 (B) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of PRDM12 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

59335

SwissProt (Human)

Q9H4Q4

Available Sizes

Frequently Asked Questions

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