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Rabbit anti-DOK4 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to DOK4

CAS Number

9007-83-4

Synonyms

Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5

Host

Rabbit

Reactivity

H, M, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human DOK4. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of DOK4 expression in HEK293T (A), HepG2 (B), rat liver (C), mouse kidney (D) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of DOK4 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

55715

Gene ID (Mouse)

114255

SwissProt (Human)

Q8TEW6

SwissProt (Mouse)

Q99KE3

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