Rabbit anti-JIP1 (pT103) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to JIP1 (pT103)
CAS Number
9007-83-4
Synonyms
IB1; JIP1; PRKM8IP; C-Jun-amino-terminal kinase-interacting protein 1; JIP-1; JNK-interacting protein 1; Islet-brain 1; IB-1; JNK MAP kinase scaffold protein 1; Mitogen-activated protein kinase 8-interacting protein 1
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human JIP1 with a phosphorylation site at T103. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH, IF/IC
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of JIP1 (pT103) expression in mouse brain (A), mouse kidney (B), rat brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of JIP1 (pT103) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
IF/ICC description
Immunofluorescent analysis of JIP1 (pT103) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Gene ID (Human)
9479
Gene ID (Mouse)
19099
Gene ID (Rat)
116457
SwissProt (Human)
Q9UQF2
SwissProt (Mouse)
Q9WVI9
SwissProt (Rat)
Q9R237
Available Sizes
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