Rabbit anti-PKC theta (pT538) Antibody
Product Specifications
Background
Rabbit polyclonal antibody to PKC theta (pT538)
CAS Number
9007-83-4
Synonyms
PRKCT; Protein kinase C theta type; nPKC-theta
Host
Rabbit
Reactivity
H, M, R
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the center region of human PKC theta with a phosphorylation site at T538. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of PKC theta (pT538) expression in mouse muscle (A), rat muscle (B) whole cell lysates.
IHC description
Immunohistochemical analysis of PKC theta (pT538) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
5588
Gene ID (Mouse)
18761
SwissProt (Human)
Q04759
SwissProt (Mouse)
Q02111
SwissProt (Rat)
Q9WTQ0
Available Sizes
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