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Rabbit anti-GPR146 Antibody

Product Specifications

Background

Rabbit polyclonal antibody to GPR146

CAS Number

9007-83-4

Synonyms

PGR8; Probable G-protein coupled receptor 146; G-protein coupled receptor PGR8

Host

Rabbit

Reactivity

H, R

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of human GPR146. The exact sequence is proprietary.

Clonality

Polyclonal

Applications

WB, IF/IC

Purification Method

The antibody was purified by immunogen affinity chromatography.

Concentration

1mg/mL

Dilution

WB (1/500 - 1/1000), IF/IC (1/50 - 1/200)

Buffer

Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Storage Conditions

Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

WB Description

Western blot analysis of GPR146 expression in A549 (A), Hela (B), rat lung (C) whole cell lysates.

IF/ICC description

Immunofluorescent analysis of GPR146 staining in LOVO cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Gene ID (Human)

115330

SwissProt (Human)

Q96CH1

Available Sizes

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