Rabbit anti-ECM1 Antibody
Product Specifications
Background
Rabbit polyclonal antibody to ECM1
CAS Number
9007-83-4
Synonyms
Extracellular matrix protein 1; Secretory component p85
Host
Rabbit
Reactivity
H, M
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human ECM1. The exact sequence is proprietary.
Clonality
Polyclonal
Applications
WB, IH
Purification Method
The antibody was purified by immunogen affinity chromatography.
Concentration
1mg/mL
Dilution
WB (1/500 - 1/1000), IH (1/50 - 1/200)
Buffer
Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Storage Conditions
Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
WB Description
Western blot analysis of ECM1 expression in MCF7 (A), A375 (B), mouse brain (C) whole cell lysates.
IHC description
Immunohistochemical analysis of ECM1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) . The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Gene ID (Human)
1893
Gene ID (Mouse)
13601
SwissProt (Human)
Q16610
SwissProt (Mouse)
Q61508
Available Sizes
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